DNA

Whole genome sequencing

Whole-genome sequencing is the most comprehensive method for analyzing the genome. It provides a high-resolution, base-by-base view of the whole genome. It can capture both common and rare variants that might otherwise be missed. It is ideal for discovery applications, such as identifying causative variants and novel genome assembly. Whole-genome sequencing can detect single nucleotide variants, insertions/deletions, copy number changes, and large structural variants.

Whole exome sequencing

Exome sequencing is one of the most widely used comprehensive targeted sequencing methods. The exome (protein-coding) represents less than 2% of the genome, but it is estimated to contain ~85% of the known disease-related variants. This makes whole-exome sequencing a cost-effective alternative to whole-genome sequencing.

Custom Targeted sequencing

Targeted sequencing, allows isolating and sequencing a subset of genes or regions of the genome. This helps us to focuses on regions of interest, generating smaller and more manageable data sets. Also reduces the sequencing costs and data analysis burdens. Reduces turnaround time and enables deep sequencing at high coverage levels for rare variant identification.

Microbe and Microbiome sequencing

NGS enables fast, accurate characterization of any species. Experimental strains, genomes with high plasticity which involves bacterium, virus, or other microbe. Sequencing small microbial genomes can be useful for contaminated foods, infectious disease and epidemiology studies etc.
Human microbiome means the microbes that are found in and on the human body. The goal of human microbiome sequencing studies is to understand the role of microbes in health and disease.

De novo sequencing

We also sequence unknown genomes and genomes with no reference sequence. De novo sequencing refers to sequencing a novel genome where there is no reference sequence available.

RNA

Reference RNA sequencing

RNA-Seq is highly sensitive and accurate tool for measuring expression across the transcriptome. It allows researchers to detect both known and novel transcript isoforms, gene fusions, single nucleotide variants, allele-specific gene expression and other features without the limitation of prior knowledge. It enables more sensitive and accurate measurement of gene expression compare to traditional methods and can be applied to any species. The price per sample turns out to be lower than many arrays.

Small RNA sequencing

Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species, such as microRNAs (miRNAs). With small RNA-Seq you can discover novel miRNAs and other small noncoding RNAs, and examine the differential expression of all small RNAs in any sample. We can understand how post-transcriptional regulation contributes to phenotype. It is also possible to identify novel biomarkers.

Epigenomes sequencing

Bisulfite- sequencing

With whole genome bisulfite sequencing, it is possible to view methylation at practically every cytosine in the genome across most species with small amounts of DNA. With targeted methylation sequencing, we can focus on regions of interest and reduce the sequencing costs and turnaround time.

Chip- sequencing

ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins.